GSM2538252: T5; Mus musculus; RNA-Seq - SRA

SRX2645715: GSM2538252: T5; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 49.2M spots, 2G bases, 1.4Gb downloads

Submitted by: NCBI (GEO)

Study: Temperature adaptation effects on BAT metabolism

PRJNA379364 • SRP101986 • All experiments • All runs

show Abstracthide Abstract

We are reporting here the effects of adaptation to different ambient temperatures in the whole genome gene expression of interscapular BAT Overall design: B6/J mice were adapted to three different ambient temperatures (30ºC, 22ºC and 6ºC) for a period of 4 weeks.

Sample: T5

SAMN06606444 • SRS2051058 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Tissues were disrupt using Tysseu lyser and Qiazol reagent. Further total RNA isolation was done using the RNeasy Quiagen Kit. Contaminating DNA was eliminated with DNA-Free kit from Ambion. RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM2538252

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 49.2M spots, 2G bases, 1.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR5349268	49,201,764	2G	1.4Gb	2017-11-15

ID:: 3825665

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